Презентация на тему: The Government Of St. Petersburg. health Committee. St. Petersburg state budget

The Government Of St. Petersburg. health Committee. St. Petersburg state budget professional educational institution " Medical College №3»
Taking material
Fixation
Washing
Dehydration
Impregnation
Casting
Block sticker
Obtaining slices on a microtome
Staining of sections and closing in optically transparent walls
Microscopy
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Первый слайд презентации: The Government Of St. Petersburg. health Committee. St. Petersburg state budget professional educational institution " Medical College №3»

Preparedstudent gr. L-31.:Dmitry Plamadyala, Sytov Alexey, Berezan Ellina The Government Of St. Petersburg. health Committee. St. Petersburg state budget professional educational institution " Medical College №3» Discipline: "Histology with histological technique»Topic : "preparation of histological preparations»

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Слайд 2: Taking material

Taken for subsequent histological examination for diagnostic purposes. The material then register.

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Слайд 3: Fixation

It is necessary to prevent the processes of decomposition, preserving the structures of life. Usually use 10% formalin solution.

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Слайд 4: Washing

Getting rid of the excess retainer and precipitation of the fixing liquid. Is on the unit wiring histological carousel type.

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Слайд 5: Dehydration

Wiring seals tissue, which is necessary for obtaining slices (if the fabric is too soft, when microcomedone it will "wrinkle" forming folds, breaks, and other artifacts, making it unusable to the study).

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Слайд 6: Impregnation

Is made to remove material from the alcohol, to further fill in paraffin and celloidin.

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Слайд 7: Casting

It is necessary for long-term archiving and obtaining slices on the microtome. Use special shapes to shape the block.

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Слайд 8: Block sticker

Blocks are glued to the wooden blocks to obtain sections on the sled microtome and further preservation in the archive.

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Слайд 9: Obtaining slices on a microtome

This is a mechanical device for obtaining histological sections. On the sled microtome, slices of paraffin and celloidin blocks are prepared. Consists of: bed limiters, micrometer, objectattributes and knife sled.

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Слайд 10: Staining of sections and closing in optically transparent walls

For the preparation of sections for painting, they remove the wax or celloidin. If the cut is obtained on the freezing microtome, it is immediately placed in the dye. Then, in the optically transparent medium.

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Слайд 11: Microscopy

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