Презентация на тему: BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1

BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1
1/51
Средняя оценка: 4.6/5 (всего оценок: 39)
Код скопирован в буфер обмена
Скачать (3893 Кб)
1

Первый слайд презентации

BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1

Изображение слайда
2

Слайд 2

LECTURE No. 1 BIOPROCESS AND ENZYME TECHNOLOGIES 2

Изображение слайда
3

Слайд 3

Biotechnology The integrated use of biochemistry, microbiology and engineering sciences to achieve technological (industrial) application capabilities of microorganisms, cultured tissue cells and parts thereof. 3 I ntroduction

Изображение слайда
4

Слайд 4

I ntroduction ¨New¨ biotechnology (methods of genetic modification and traditional biotechnology) Traditional biotechnology ( conventional techniques ) 4

Изображение слайда
5

Слайд 5

Introduction Main areas of biotechnology Bioprocess technology Enzyme technology Waste technology Environmental technology Renewable resources technology Plant and animal agriculture Healthcare 5

Изображение слайда
6

Слайд 6

Introduction White Green Blue Gray Red 6 Biotechnology

Изображение слайда
7

Слайд 7

7 Industrial supplies ( biochemicals, enzymes and reagents for industrial and food processing); Environment (pollution diagnostics, products for pollution prevention, bioremediation ); Energy (fuels from renewable resources ). Introduction

Изображение слайда
8

Слайд 8

8 Резюме Актуальные проблемы химии Биотехнология, это изпользование микробных, животных или растительных клеток или ферментов для синтеза, разщепления или преобразования материалов. Области биотехнологии промышленные товары окружающая обстановка энергия

Изображение слайда
9

Слайд 9

Objectives To discuss general aspects of bioprocesses considering: P rinciples of microbial growth C haracteristics of large-scale fermentation Isolation and purification F ermentation scale up 9 To describe the general characteristics of enzymes

Изображение слайда
10

Слайд 10

Features of an industrial microorganism : Capable to grow and produce the substance of interest in large scale cultivation To grow rapidly and produce the desired product in short time ( productivity ) Able to grow in an inexpensive liquid culture medium It should not be pathogenic Susceptible to genetic manipulation 10 Principles of microbial growth

Изображение слайда
11

Слайд 11

11 Examples of bioproducts : Similar product formation steps

Изображение слайда
12

Слайд 12

12 A schematic representation of fermentation process

Изображение слайда
13

Слайд 13

M icrobial growth Growth depends on: availability and transport of necessary nutrients to the cell environmental parameters (temperature, pH and aeration) 13 Definition : the increase of cell material expressed in terms of mass or cell number. Measurement of formed biomass : gravimetrically or numerically

Изображение слайда
14

Слайд 14

- S: concentration of a substrate in the medium Monod equation (µ: specific growth) - µmax: maximum specific growth rate of the organism - Ks: saturation constant 14 M icrobial growth

Изображение слайда
15

Слайд 15

Main ways of growing microorganisms: in liquid or solid conditions 15 static or agitated cultures in the presence or absence of oxygen free or attached to surfaces

Изображение слайда
16

Слайд 16

Main ways of growing microorganisms: Batch: constant volume, continuous consumption of nutrients. 16

Изображение слайда
17

Слайд 17

Main ways of growing microorganisms: Fed-batch: addition of nutrients to prevent nutrient depletion. The formed product remains in the bioreactor. 17

Изображение слайда
18

Слайд 18

Main ways of growing microorganisms: Continuous: steady state (unchanging). Continuously addition of fresh medium and removing of formed product and residues. 18

Изображение слайда
19

Слайд 19

19 Microbial growth in batch culture Lag phase Exponential phase (max rate of growth, nutrients in excess, ideal environmental parameters) Stationary phase Death phase

Изображение слайда
20

Слайд 20

20 P rimary metabolite Secondary metabolite Types of microbial metabolites

Изображение слайда
21

Слайд 21

21 Резюме Зависит от: - доступность и транспортировка необходимых пытательных веществ в клетку. - параметры окружающей среды (Т, рН, аэрация) - статические или перемешанные культуры - при наличи или отсуствии кислорода - в жидком или твердом состояние - свободные или прикрепленные к поверхностям Рост Происходит :

Изображение слайда
22

Слайд 22

22 Резюме - партия - поданная партия - неприрывно Рост Происходит : П роизводит - первичные - вторичные

Изображение слайда
23

Слайд 23

Characteristics of large-scale fermentation. The bioreactor simple stirred or non-stirred open containers complex aseptic integrated systems batch, fed-batch and continuous manner 23

Изображение слайда
24

Слайд 24

Characteristics of large-scale fermentation. The bioreactor From 5 to 10 L (laboratory scale) and up to 500 000 L (industrial scale) Anaerobic: more simple and requires removal of heat generated during fermentation. Aerobic: more complex and requires a good mixing and aeration system. 24

Изображение слайда
25

Слайд 25

25 Yield: amount of obtained product. Productivity: rate of product formation.

Изображение слайда
26

Слайд 26

Bioreactor control measurements S pecific growth Environmental parameters 26 Temperature pH Oxygen concentration Cell mass Levels of nutrients Product concentration

Изображение слайда
27

Слайд 27

Bioreactor control measurements The data can be collected: on-line (directly): is available for temperature, pH, dissolved oxygen and carbon dioxide analyses off-line: a sample is removed aseptically from the process stream and analyzed M edia design : source of carbon, nitrogen, phosphorous, vitamins, salts, antifoam agents 27

Изображение слайда
28

Слайд 28

28 Isolation and purification - Separation ( filtration, centrifugation, flotation, disruption ) - Concentration (solubilisation, extraction, thermal processing, membrane filtration, precipitation ) - Purification ( crystallization, chromatography ) - Modification Product recovery and purification : 70-80% of final cost - Drying

Изображение слайда
29

Слайд 29

Fermentation scale-up Laboratory flask Laboratory reactor (1-10 L) Pilot plant stage (300 - 3 000 L) Commercial reactor (10 000 - 500 000 L) Mixing and aeration 29

Изображение слайда
30

Слайд 30

Fermentation scale-up The management of scale -up requieres high capital investment in: Mixing and aeration Monitoring and controlled devices Maintenance of sterility 30

Изображение слайда
31

Слайд 31

31 Резюме анаэробные аэробные процессы партия поданная партия неприрывно Реакторы Важные параметры - рост - формирование продукта Питательная среда : углерод азот вода Разделение и очистка Масштабированный

Изображение слайда
32

Слайд 32

32 Biocatalytic processes Biocatalysis The use of cells or their isolated enzymes to catalyze reactions or transformations that lead to obtaining compounds of interest.

Изображение слайда
33

Слайд 33

Importance of biocatalytic process It represents a more efficient and at the same time more ecological alternative to traditional synthetic chemistry. The processes pass through reactions in more friendly environments and under mild pH and temperature conditions with higher yields. 33 Biocatalytic processes

Изображение слайда
34

Слайд 34

34 Industrial supplies ( biochemicals, enzymes and reagents for industrial and food processing); Environment (pollution diagnostics, products for pollution prevention, bioremediation ); Energy (fuels from renewable resources ). Categories of Biotechnology

Изображение слайда
35

Слайд 35

35 Enzymes. D efinition and role accelerate the speed of chemical reactions that occur in a cell " biological catalysts " highly specific, work in mild conditions, are easily available and preserve the environment Enzymes are a special class of proteins General characteristics of enzymes

Изображение слайда
36

Слайд 36

36 Classification of the enzymes Enzyme class Reaction type Oxidoreductases Transferases Hydrolases Lyases

Изображение слайда
37

Слайд 37

37 Classification of the enzymes Enzyme class Reaction type Isomerases Ligases

Изображение слайда
38

Слайд 38

38 Mode of action

Изображение слайда
39

Слайд 39

39 Mode of action

Изображение слайда
40

Слайд 40

40 Factors affecting catalytic activity of enzymes C oncentration V [E] V [S] Enzyme and substrate concentrations Substrate concentration Fig. A Fig. B

Изображение слайда
41

Слайд 41

41 Factors affecting catalytic activity of enzymes Substrate concentration Fig. A Fig. B

Изображение слайда
42

Слайд 42

42 Factors affecting catalytic activity of enzymes Substrate concentration Michaelis-Menten constant Low affinity High affinity K m >>>, [E] [S ] K m <<<, [ES ] K m = S

Изображение слайда
43

Слайд 43

43 Factors affecting catalytic activity of enzymes Substrate concentration K m is the required substrate concentration to reach :

Изображение слайда
44

Слайд 44

44 Factors affecting catalytic activity of enzymes Substrate concentration Enzyme efficiency : = 10 8 – 10 9 mol s -1 Enzyme catalytically perfect

Изображение слайда
45

Слайд 45

45 Temperature Factors affecting catalytic activity of enzymes

Изображение слайда
46

Слайд 46

46 pH Factors affecting catalytic activity of enzymes

Изображение слайда
47

Слайд 47

47 Резюме оксидоредуктазы трансферазы гидролазы лиасы изомеразы лигазы Ферменты ферментативная активность Температура pH концентрация

Изображение слайда
48

Слайд 48

Control questions 1. Why Industrial Microbiology is important? 2. Mention the main characteristics of an industrial microorganism. 3. Explain why ethanol is a primary metabolite. 4. Indicate some environmental factors that should be controlled during fermentation process. Explain how the variation of one of them can affect the process. 5. Discuss scale-up and the importance of fermentation scale-up. 6. Mention some examples of microbial products. 48

Изображение слайда
49

Слайд 49

Control questions (continued) 7. Define the biocatalytic process and explain its importance. 8. What is the nature of an enzyme? Explain its mode of action. Consider the activation energy and the most important parameters on which the enzymatic activity depends. 49

Изображение слайда
50

Слайд 50

50 - Smith J.E.Biotechnology. Fifth Edition, 2009, 280 p. ISBN-13 978-0-511-46394-5 - Stanbury P.F., Whitaker A., Hall S.J. Principles of fermentation technology. 2nd Edition, 1995, Elsevier Science Ltd., 367 p. ISBN 0 7506 4501 6. - Robinson P.K. Enzymes: principles and biotechnological applications. Essays Biochem. (2015) 59, 1–41: doi : 10.1042/BSE0590001. - Smith J.E. Biotechnology. Fifth Edition, 2009, 280 p. ISBN-13 978-0-511-46394-5. Suggested literature

Изображение слайда
51

Последний слайд презентации: BIOPROCESS TECHNOLOGY Dr. TERESA FERNANDEZ ALDAMA “SAMARA UNIVERSITY” 1

THANK YOU FOR YOUR ATTENTION 51

Изображение слайда